Characterization of a gene involved in histidine biosynthesis in Halobacterium (Haloferax) volcanii: isolation and rapid mapping by transformation of an auxotroph with cosmid DNA.

Abstract excerpt

Techniques for the transformation of halophilic archaebacteria have been developed recently and hold much promise for the characterization of these organisms at the molecular level. In order to understand genome organization and gene regulation in halobacteria, we have begun the characterization of genes involved in amino acid biosynthesis in Halobacterium (Haloferax) volcanii. These studies are facilitated by...

Topics

• Amino Acid Sequence
• Base Sequence
• Chromosome Mapping
• DNA, Bacterial
• Genes, Bacterial
• Halobacterium
• Histidine
• Molecular Sequence Data
• Mutation
• Transaminases
• Transformation, Bacterial