Construction and analysis of a recombination-deficient (radA) mutant of Haloferax volcanii.

doi:10.1046/j.1365-2958.1997.2651626.x

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Article

Construction and analysis of a recombination-deficient (radA) mutant of Haloferax volcanii.

Molecular microbiology - 1 Feb 1997

Woods W G, Dyall-Smith M L

Abstract excerpt

By deleting the radA open reading frame of an extreme halophile, Haloferax volcanii, we created and characterized a recombination-deficient archaeon. This strain, Hf. volcanii DS52, has no detectable DNA recombination, is more sensitive to DNA damage by UV light and ethylmethane sulfonate, and ha...

Topics

Archaeal Proteins
Bacterial Proteins
Base Sequence
DNA Primers
DNA Repair
DNA Replication
DNA, Bacterial
DNA-Binding Proteins
Gene Deletion
Halobacteriales
Mutation
Plasmids
Recombination, Genetic

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