Article
Construction and analysis of a recombination-deficient (radA) mutant of Haloferax volcanii.
Molecular microbiology - 1 Feb 1997
Woods W G, Dyall-Smith M L
Abstract excerpt
By deleting the radA open reading frame of an extreme halophile, Haloferax volcanii, we created and characterized a recombination-deficient archaeon. This strain, Hf. volcanii DS52, has no detectable DNA recombination, is more sensitive to DNA damage by UV light and ethylmethane sulfonate, and has a slower growth rate than the wild type. These characteristics are similar to those observed in recombination mutants...
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