Article
Phenotyping in the archaea: optimization of growth parameters and analysis of mutants of Haloferax volcanii.
FEMS microbiology letters - 1 Sept 2011
Jantzer Katharina, Zerulla Karolin, Soppa Jörg
Abstract excerpt
A method to grow the halophilic archaeon Haloferax volcanii in microtiter plates has been optimized and now allows the parallel generation of very reproducible growth curves. The doubling time in a synthetic medium with glucose is around 6 h. The method was used to optimize glucose and casamino acid concentrations, to clarify carbon source usage and to analyze vitamin dependence. The characterization of...
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