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Article

Development of a gene knockout system for the halophilic archaeon Haloferax volcanii by use of the pyrE gene.

Journal of bacteriology - 1 Feb 2003

Bitan-Banin Gili, Ortenberg Ron, Mevarech Moshe

Abstract excerpt

So far, the extremely halophilic archaeon Haloferax volcanii has the best genetic tools among the archaea. However, the lack of an efficient gene knockout system for this organism has hampered further genetic studies. In this paper we describe the development of pyrE-based positive selection and counterselection systems to generate an efficient gene knockout system. The H. volacanii pyrE1 and pyrE2 genes were...

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Identifiers

PMID: 12533452
DOI: 10.1128/JB.185.3.772-778.2003
PMCID: PMC142808

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