Article
Reduced mRNA expression in paraffin-embedded tissue identifies MLH1- and MSH2-deficient colorectal tumours and potential mutation carriers.
Virchows Archiv : an international journal of pathology - 1 Jul 2008
Müller Annegret, Zielinski Dirk, Friedrichs Nicolaus, Oberschmid Barbara, Merkelbach-Bruse Sabine, Schackert Hans K, Linnebacher Markus, von Knebel Doeberitz Magnus, Büttner Reinhard, Rüschoff Josef
Abstract excerpt
Based on the principle of nonsense-mediated mRNA decay, we sought to identify MLH1 or MSH2-deficient colorectal tumours through relative quantification of mRNA expression with real-time PCR (RT-PCR) analysis. MLH1 and MSH2 mRNAs were almost equally expressed as defined by MLH1 to MSH2 transcript ratio (mean 1.41) in microsatellite stable, mismatch repair (MMR) proficient tumours (n = 16). A close correlation...
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