Article
Turnover of endogenous SsrA-tagged proteins mediated by ATP-dependent proteases in Escherichia coli.
The Journal of biological chemistry - 22 Aug 2008
Lies Mark, Maurizi Michael R
Abstract excerpt
Formation and degradation of SsrA-tagged proteins enable ribosome recycling and elimination of defective products of incomplete translation. We produced an antibody against the SsrA peptide and used it to measure the amounts of SsrA-tagged proteins in Escherichia coli cells without interfering with tagging or altering the context of the tag added at the ends of nascent polypeptides. SsrA-tagged proteins were...
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