Article
MLH1 deficiency enhances radiosensitization with 5-fluorodeoxyuridine by increasing DNA mismatches.
Molecular pharmacology - 1 Sept 2008
Flanagan Sheryl A, Krokosky Christina M, Mannava Sudha, Nikiforov Mikhail A, Shewach Donna S
Abstract excerpt
The antitumor drug 5-fluoro-2'-deoxyuridine (FdUrd) also sensitizes tumor cells to ionizing radiation in vitro and in vivo. Although radiosensitization with FdUrd requires dTTP depletion and S-phase arrest, the exact mechanism by which these events produce radiosensitization remains unknown. We hypothesized that the depletion of dTTP produces DNA mismatches that, if not repaired before irradiation, would result...
Topics
- Adaptor Proteins, Signal Transducing
- Base Pair Mismatch
- Cell Cycle
- Cell Death
- Floxuridine
- HCT116 Cells
- Humans
- MutL Protein Homolog 1
- Mutation
- Nuclear Proteins
- Nucleotides
- Plasmids
- RNA, Small Interfering
- Radiation Tolerance
- Radiation, Ionizing