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Article

Influence of the isolation method on the stability of differentiated phenotype in cultured rat hepatocytes.

Journal of pharmacological methods - 1 Mar 1991

Bayad J, Sabolovic N, Bagrel D, Magdalou J, Siest G

Abstract excerpt

Primary cultures of adult rat hepatocytes were established using two different isolation procedures: a two-step collagenase perfusion and a method using ethylenediaminetetraacetate (EDTA) as the dissociating agent. Both techniques provided good yields of hepatocytes with comparable viability. The evolution of hepato-specific protein levels and several drug-metabolizing enzyme activities were followed for 8 days...

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PMID: 1851520
DOI: 10.1016/0160-5402(91)90025-z

Genes

TAT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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