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Article

Influence of the isolation method on the stability of differentiated phenotype in cultured rat hepatocytes.

Journal of pharmacological methods - 1 Mar 1991

Bayad J, Sabolovic N, Bagrel D, Magdalou J, Siest G

Abstract excerpt

Primary cultures of adult rat hepatocytes were established using two different isolation procedures: a two-step collagenase perfusion and a method using ethylenediaminetetraacetate (EDTA) as the dissociating agent. Both techniques provided good yields of hepatocytes with comparable viability. The...

Topics

Animals
Cell Separation
Cell Survival
Edetic Acid
Liver
Male
Microbial Collagenase
Phenotype
Proto-Oncogenes
Rats
Rats, Inbred Strains

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1851520
DOI: 10.1016/0160-5402(91)90025-z

Genes

TAT

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1851520-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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