Article
Long-term cultivation of adult rat hepatocytes that undergo multiple cell divisions and express normal parenchymal phenotypes.
The American journal of pathology - 1 Feb 1996
Tateno C, Yoshizato K
Abstract excerpt
The present study succeeded in cultivating normal adult rat hepatocytes for at least 85 days without losing their replicative potential and differentiation capacity. Small pieces of hepatocyte aggregates (clusters) were prepared from the primary culture of hepatocytes and used as starting materia...
Topics
- Albumins
- Animals
- Ascorbic Acid
- Cell Aggregation
- Cell Culture Techniques
- Cell Division
- Culture Media
- Dimethyl Sulfoxide
- Epidermal Growth Factor
- Immunohistochemistry
- Liver
- Male
- Microscopy, Electron
- Niacinamide
- Phenotype
- Rats
- Rats, Inbred F344
- Time Factors