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Article

Extra collagen overlay prolongs the differentiated phenotype in sandwich-cultured rat hepatocytes.

Journal of pharmacological and toxicological methods - 1 Jan 2000

Oorts Marlies, Keemink Janneke, Deferm Neel, Adriaensen Robin, Richert Lysiane, Augustijns Patrick, Annaert Pieter

Abstract excerpt

INTRODUCTION: Sandwich-cultured rat hepatocytes (SCRH) have become an invaluable in vitro model to study hepatic drug disposition. SCRH are maintained between two layers of extracellular matrix. In this configuration, culture periods of 4days are typically applicable. The aim of the present study was to modify conventional SCRH by applying an additional collagen overlay to prolong the hepatic phenotype in SCRH...

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Identifiers

PMID: 29079456
DOI: 10.1016/j.vascn.2017.10.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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