Article

Isolation and Primary Culture of Rat Hepatic Cells

2012-06-29

Abstract excerpt

Primary hepatocyte culture is a valuable tool that has been extensively used in basic research of liver function, disease, pathophysiology, pharmacology and other related subjects. The method based on two-step collagenase perfusion for isolation of intact hepatocytes was first introduced by Berry and Friend in 1969 1 and, since then, has undergone many modifications. The most commonly used technique was described...

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Literature Corpus work: 670ed87b-72f0-5c16-8628-937195a758fc
DOI: 10.3791/3917-v

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