Production of a heterozygous mutant cell line by homologous recombination (single knockout).

doi:10.1002/0471142301.ns0430s21

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Article

Production of a heterozygous mutant cell line by homologous recombination (single knockout).

Current protocols in neuroscience - 1 Feb 2003

Mortensen Richard

Abstract excerpt

Gene targeting by homologous recombination allows the introduction of specific mutations into any cloned gene. This unit provides a protocol in which the gene of interest is inactivated by interrupting its coding sequence with a positive selectable marker. A negative selectable marker is included in the construct outside the region of target gene homology in order to enrich for clones in which the target gene has...

Topics

Animals
Biomedical Research
Cell Line
Embryonic Stem Cells
Gene Deletion
Gene Expression Regulation, Enzymologic
Gene Targeting
Heterozygote
Humans
Integrases
Mutation
Neurosciences
Recombination, Genetic

Source

Open in PubMed

Identifiers

PMID: 18428579
DOI: 10.1002/0471142301.ns0430s21

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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