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Article

Production of a heterozygous mutant cell line by homologous recombination (single knockout).

Current protocols in molecular biology - 1 May 2001

Mortensen R

Abstract excerpt

Formerly UNIT 9.16, this unit takes a more appropriate place in Chapter 23, and has been updated and revised for this publication. Gene targeting by homologous recombination allows the introduction of specific mutations into any cloned gene. In this unit, the gene of interest is inactivated by interrupting its coding sequence with a positive selectable marker (e.g., neo). Expression of neo is obtained by...

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PMID: 18265206
DOI: 10.1002/0471142727.mb2305s52

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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