Article
Genetic knockouts and knockins in human somatic cells.
Nature protocols - 1 Jan 2007
Rago Carlo, Vogelstein Bert, Bunz Fred
Abstract excerpt
Gene targeting by homologous recombination with exogenous DNA constructs is the most powerful technique available for analysis of mammalian gene function. Over the past several years, the methods used to generate knockout and knockin mice have been modified for use in cultured human cells. The most significant innovation has been the adaptation of recombinant adeno-associated viruses (rAAVs) for such targeting....
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