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Article

Transfer of YAC clones to new hosts by karyogamy-deficient mating.

Current protocols in human genetics - 1 May 2001

Spencer F, Simchen G

Abstract excerpt

This unit provides a protocol for moving yeast artificial chromosome (YAC) clones to new yeast hosts using basic microbial techniques and pulsed-field gel analysis. In contrast to other methods that can be used to transfer YAC clones, this requires neither optimization to achieve high-efficiency DNA-mediated transformation of chromosome-sized DNA nor specialized equipment for tetrad dissection and analysis....

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PMID: 18428288
DOI: 10.1002/0471142905.hg0514s06

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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