Article
A versatile method for efficient YAC transfer between any two strains.
Genomics - 1 Jul 1994
Hugerat Y, Spencer F, Zenvirth D, Simchen G
Abstract excerpt
The ability to transfer yeast artificial chromosome (YAC) clones among yeast hosts greatly enhances their utility as cloned DNAs by increasing the range of methods available for experimental manipulation. An effective method for the transfer of YACs between strains in Kar1- matings is described in the accompanying paper (F. Spencer et al., 1994, Genomics 22, 118-126). To evaluate the general nature of the new...
Topics
- Animals
- Chromosomes, Artificial, Yeast
- Cloning, Molecular
- Gene Transfer Techniques
- Genes, Fungal
- Genes, Mating Type, Fungal
- Genetic Techniques
- Genetic Vectors
- Humans
- Mutation
- Saccharomyces cerevisiae
