Article
The COP9/signalosome increases the efficiency of von Hippel-Lindau protein ubiquitin ligase-mediated hypoxia-inducible factor-alpha ubiquitination.
The Journal of biological chemistry - 13 Jun 2008
Miyauchi Yasuhiro, Kato Michiko, Tokunaga Fuminori, Iwai Kazuhiro
Abstract excerpt
Oxygen-dependent ubiquitination of the alpha-subunit of hypoxia-inducible factor (HIF-alpha) by the (von Hippel-Lindau protein)-Elongin B/C-Cullin2-Rbx1 (VBC-Cul2) ubiquitin ligase, a member of the cullin-RING ubiquitin ligases (CRLs), plays a central role in controlling oxygen metabolism. Nedd8 conjugation of cullins enhances the ligase activity of CRLs, and the COP9/signalosome (CSN) enhances the degradation of...
Topics
- COP9 Signalosome Complex
- Cell Line, Tumor
- Gene Expression Regulation
- HeLa Cells
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
- Kinetics
- Models, Biological
- Multiprotein Complexes
- Mutation
- Peptide Hydrolases
- Plasmids
- Protein Binding
- RNA Interference