Article
Identification of Elongin C and Skp1 sequences that determine Cullin selection.
The Journal of biological chemistry - 8 Oct 2004
Yan Qin, Kamura Takumi, Cai Yong, Jin Jingji, Ivan Mircea, Mushegian Arcady, Conaway Ronald C, Conaway Joan Weliky
Abstract excerpt
The multiprotein von Hippel-Lindau (VHL) tumor suppressor and Skp1-Cul1-F-box protein (SCF) complexes belong to families of structurally related E3 ubiquitin ligases. In the VHL ubiquitin ligase, the VHL protein serves as the substrate recognition subunit, which is linked by the adaptor protein Elongin C to a heterodimeric Cul2/Rbx1 module that activates ubiquitylation of target proteins by the E2...
Topics
- Amino Acid Sequence
- Anaphase-Promoting Complex-Cyclosome
- Animals
- Binding Sites
- Blotting, Western
- Cell Line
- Cullin Proteins
- Elongin
- Esters
- F-Box Proteins
- Humans
- Hypoxia-Inducible Factor 1, alpha Subunit
- Immunoprecipitation