Article
A robust toolkit for functional profiling of the yeast genome.
Molecular cell - 5 Nov 2004
Pan Xuewen, Yuan Daniel S, Xiang Dong, Wang Xiaoling, Sookhai-Mahadeo Sharon, Bader Joel S, Hieter Philip, Spencer Forrest, Boeke Jef D
Abstract excerpt
Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAGs and the SGA reporter, MFA1pr-HIS3, which facilitates conversion of heterozygous diploid YKO...
Read the complete abstract on PubMedTopics
- Gene Expression Profiling
- Gene Expression Regulation, Fungal
- Genome, Fungal
- Internet
- Mutation
- Oligonucleotide Array Sequence Analysis