Article
Temporal control of gene silencing by in ovo electroporation.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Baeriswyl Thomas, Mauti Olivier, Stoeckli Esther T
Abstract excerpt
The analysis of gene function during embryonic development asks for tight temporal control of gene expression. Classic genetic tools do not allow for this, as the absence of a gene during the early stages of development will preclude its functional analysis during later stages. In contrast, RNAi technology allows one to achieve temporal control of gene silencing especially when used with oviparous animal models....
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