Article
In ovo electroporation of miRNA-based-plasmids to investigate gene function in the developing neural tube.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Andermatt Irwin, Wilson Nicole, Stoeckli Esther T
Abstract excerpt
When studying gene function in vivo during development, gene expression has to be controlled in a precise temporal and spatial manner. Technologies based on RNA interference (RNAi) are well suited for such studies, as they allow for the efficient silencing of a gene of interest. In contrast to challenging and laborious approaches in mammalian systems, the use of RNAi in combination with oviparous animal models...
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