Article
Time-lapse Confocal Imaging of Migrating Neurons in Organotypic Slice Culture of Embryonic Mouse Brain Using In Utero Electroporation.
Journal of visualized experiments : JoVE - 25 Jul 2017
Wiegreffe Christoph, Feldmann Svenja, Gaessler Simeon, Britsch Stefan
Abstract excerpt
In utero electroporation is a rapid and powerful approach to study the process of radial migration in the cerebral cortex of developing mouse embryos. It has helped to describe the different steps of radial migration and characterize the molecular mechanisms controlling this process. To directly and dynamically analyze migrating neurons they have to be traced over time. This protocol describes a workflow that...
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