Article
Ex ovo electroporation of early chicken embryos.
STAR protocols - 18 Jun 2021
Williams Ruth M, Sauka-Spengler Tatjana
Abstract excerpt
The chick embryo is a favored model for developmental studies owing to its accessibility and ease of manipulation. Ex ovo electroporation provides a highly efficient method for screening perturbation phenotypes using a variety of reagents, including CRISPR and morpholinos. Additionally, the chick system lends itself well to rapid medium-throughput enhancer screening. Constructs facilitating tissue-specific...
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