Article
Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst Using Plasmid Electroporation.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016
Stoller Michelle L, Fekete Donna M
Abstract excerpt
The avian embryo has a well-documented history as a model system for the study of neurogenesis, morphogenesis, and cell fate specification. This includes studies of the chicken inner ear that employ in ovo electroporation, in conjunction with the Tol2 system, to yield robust long-term transgene expression. Capitalizing on the success of this delivery method, we describe a modified version of the Tol2 expression...
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