Article
SNP-specific array-based allele-specific expression analysis.
Genome research - 1 May 2008
Bjornsson Hans T, Albert Thomas J, Ladd-Acosta Christine M, Green Roland D, Rongione Michael A, Middle Christina M, Irizarry Rafael A, Broman Karl W, Feinberg Andrew P
Abstract excerpt
We have developed an optimized array-based approach for customizable allele-specific gene expression (ASE) analysis. The central features of the approach are the ability to select SNPs at will for detection, and the absence of need to PCR amplify the target. A surprisingly long probe length (39-49 nt) was needed for allelic discrimination. Reconstitution experiments demonstrate linearity of ASE over a broad...
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