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Article

Adaptive Mutations Enhance Assembly and Cell-to-Cell Transmission of a High-Titer Hepatitis C Virus Genotype 5a Core-NS2 JFH1-Based Recombinant.

Journal of virology - 1 Aug 2015

Mathiesen Christian K, Prentoe Jannick, Meredith Luke W, Jensen Tanja B, Krarup Henrik, McKeating Jane A, Gottwein Judith M, Bukh Jens

Abstract excerpt

UNLABELLED: Recombinant hepatitis C virus (HCV) clones propagated in human hepatoma cell cultures yield relatively low infectivity titers. Here, we adapted the JFH1-based Core-NS2 recombinant SA13/JFH1C3405G,A3696G (termed SA13/JFH1orig), of the poorly characterized genotype 5a, to Huh7.5 cells, yielding a virus with greatly improved spread kinetics and an infectivity titer of 6.7 log10 focus-forming units...

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Identifiers

PMID: 25995244
DOI: 10.1128/JVI.00039-15
PMCID: PMC4505679

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