Article
Identification of the functional domains of the transcriptional regulator CRE-BP1.
The Journal of biological chemistry - 25 Sept 1991
Matsuda S, Maekawa T, Ishii S
Abstract excerpt
We present evidence that CRE-BP1 binding to the cyclic AMP (cAMP) response element (CRE) is a transcriptional activator. Transcriptional activation was assayed by cotransfection into CV-1 cells of a CRE-BP1 expression plasmid together with a reporter plasmid in which the thymidine kinase promoter...
Topics
- Amino Acid Sequence
- Blotting, Western
- Chloramphenicol O-Acetyltransferase
- Cyclic AMP Response Element-Binding Protein
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-jun
- Transcription, Genetic
- Transcriptional Activation
- Transfection