Article
E1A-mediated activation of the adenovirus E4 promoter can occur independently of the cellular transcription factor E4F.
Molecular and cellular biology - 1 Sept 1991
Jones C, Lee K A
Abstract excerpt
The cellular factors E4F and ATF-2 (a member of the activating transcription factor [ATF] family) bind to common sites in the adenovirus E4 promoter and have both been suggested to mediate transcriptional activation by the viral E1A protein. To assess the role of E4F, we have introduced mutations into the E4F/ATF binding sites of the E4 promoter and monitored promoter activity in HeLa cells. We find that the core...
Topics
- Adenovirus Early Proteins
- Base Sequence
- Binding Sites
- DNA Probes
- DNA, Viral
- Electrophoresis
- Gene Expression Regulation, Viral
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutation