Article
Still looking for the magic spot: the crystallographically defined binding site for ppGpp on RNA polymerase is unlikely to be responsible for rRNA transcription regulation.
Journal of molecular biology - 21 Mar 2008
Vrentas Catherine E, Gaal Tamas, Berkmen Melanie B, Rutherford Steven T, Haugen Shanil P, Vassylyev Dmitry G, Ross Wilma, Gourse Richard L
Abstract excerpt
Identification of the RNA polymerase (RNAP) binding site for ppGpp, a central regulator of bacterial transcription, is crucial for understanding its mechanism of action. A recent high-resolution X-ray structure defined a ppGpp binding site on Thermus thermophilus RNAP. We report here effects of ppGpp on 10 mutant Escherichia coli RNAPs with substitutions for the analogous residues within 3-4 A of the ppGpp...
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