Article
A novel genotyping strategy based on allele-specific inverse PCR for rapid and reliable identification of conditional FADD knockout mice.
Molecular biotechnology - 1 Feb 2008
Dong Xiangbai, Li Jie, Li Shufeng, Zhang Jing, Hua Zi-chun
Abstract excerpt
The apoptotic adapter protein FADD has been shown to play diverse roles in cell survival and proliferation. FADD knockout embryos died of heart defects, rendering Cre/loxP-mediated conditional FADD knockout mice a unique tool for investigating FADD-dependent nonapoptotic mechanism. Previously, these genetically engineered mice were identified by time-consuming Southern blot or controversial real-time PCR. In this...
Topics
- Alleles
- Animals
- Blotting, Southern
- Fas-Associated Death Domain Protein
- Genotype
- Mice
- Mice, Knockout
- Polymerase Chain Reaction
- Sensitivity and Specificity
- T-Lymphocytes
- Time Factors