Article
Expanding the functional repertoire of CTD kinase I and RNA polymerase II: novel phosphoCTD-associating proteins in the yeast proteome.
Biochemistry - 21 Dec 2004
Phatnani Hemali P, Jones Janice C, Greenleaf Arno L
Abstract excerpt
CTD kinase I (CTDK-I) of Saccharomyces cerevisiae is required for normal phosphorylation of the C-terminal repeat domain (CTD) on elongating RNA polymerase II. To elucidate cellular roles played by this kinase and the hyperphosphorylated CTD (phosphoCTD) it generates, we systematically searched yeast extracts for proteins that bound to the phosphoCTD made by CTDK-I in vitro. Initially, using a combination of...
Topics
- Casein Kinase I
- Methyltransferases
- Models, Molecular
- Mutation
- Peptides
- Phosphorylation
- Protein Binding
- Protein Kinases
- Protein Structure, Tertiary
- Proteome
- RNA Polymerase II
- RNA Splicing
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins