Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction.

RNA (New York, N.Y.) - 1 Dec 1997

Spinelli S L, Consaul S A, Phizicky E M

Abstract excerpt

tRNA splicing is essential in yeast and humans and presumably all eukaryotes. The first two steps of yeast tRNA splicing, excision of the intron by endonuclease and joining of the exons by tRNA ligase, leave a splice junction bearing a 2'-phosphate. Biochemical analysis suggests that removal of this phosphate in yeast is catalyzed by a highly specific 2'-phosphotransferase that transfers the phosphate to NAD to...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Genes, Fungal
Share to Genes, Lethal
Share to Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to RNA, Fungal

Share to RNA Splicing

Share to RNA, Transfer

Share to Saccharomyces cerevisiae

Share to Saccharomyces cerevisiae Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 9404890
PMCID: PMC1369580

Genes

TPT1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction.PMID 9404890Read ↗

Genetic bypass of essential RNA repair enzymes in budding yeast.Semantic proximity Read ↗

Two-step mechanism and step-arrest mutants of Runella slithyformis NAD+-dependent tRNA 2'-phosphotransferase Tpt1.Semantic proximity Read ↗

Site specific phosphorylation of yeast RNA polymerase I.Semantic proximity Read ↗

A highly specific phosphatase that acts on ADP-ribose 1''-phosphate, a metabolite of tRNA splicing in Saccharomyces cerevisiae.Semantic proximity Read ↗

A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis.Semantic proximity Read ↗

Mistranslating tRNA variants impact the proteome and phosphoproteome of <i>Saccharomyces cerevisiae</i>Semantic proximity Read ↗

Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA.Semantic proximity Read ↗

NAD+-dependent synthesis of a 5'-phospho-ADP-ribosylated RNA/DNA cap by RNA 2'-phosphotransferase Tpt1.Semantic proximity Read ↗

A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family.Semantic proximity Read ↗

Mistranslating tRNA variants impact the proteome and phosphoproteome of Saccharomyces cerevisiae.Semantic proximity Read ↗

Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae.Semantic proximity Read ↗

A mutation in a methionine tRNA gene suppresses the prp2-1 Ts mutation and causes a pre-mRNA splicing defect in Saccharomyces cerevisiae.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.