Article
Temperature-sensitive mutants of RNase E in Salmonella enterica.
Journal of bacteriology - 1 Dec 2011
Hammarlöf Disa L, Liljas Lars, Hughes Diarmaid
Abstract excerpt
RNase E has an important role in mRNA turnover and stable RNA processing, although the reason for its essentiality is unknown. We isolated conditional mutants of RNase E to provide genetic tools to probe its essential function. In Salmonella enterica serovar Typhimurium, an extreme slow-growth phenotype caused by mutant EF-Tu (Gln125Arg, tufA499) can be rescued by mutants of RNase E that have reduced activity. We...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Endoribonucleases
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Peptide Elongation Factor Tu
- RNA Processing, Post-Transcriptional
- RNA, Bacterial
- RNA, Messenger
- Salmonella typhimurium
- Temperature