Article
Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA targets by their interactions with GW182 proteins AIN-1 and AIN-2.
Molecular cell - 30 Nov 2007
Zhang Liang, Ding Lei, Cheung Tom H, Dong Meng-Qiu, Chen Jun, Sewell Aileen K, Liu Xuedong, Yates John R, Han Min
Abstract excerpt
MicroRNAs (miRNAs) regulate gene expression for diverse functions, but only a limited number of mRNA targets have been experimentally identified. We show that GW182 family proteins AIN-1 and AIN-2 act redundantly to regulate the expression of miRNA targets, but not miRNA biogenesis. Immunoprecipitation (IP) and mass spectrometry indicate that AIN-1 and AIN-2 interact only with miRNA-specific Argonaute proteins...
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