Article
Design of dual-labeled oligonucleotide probes for SNPs genotyping.
Nucleic acids symposium series (2004) - 1 Jan 2007
Saito Yoshio, Bag Subhendu Sekhar, Kodate Satoshi, Suzuka Isamu
Abstract excerpt
Our effort in designing base-discriminating fluorescence nucleosides (BDF), leads us to develop dual-labeled oligonucleotide probe in which the BDF nucleoside, (Py)U/(2-Ant)U act as the donor separated by a defined base pair distance from the acceptor, fluorescein, attached to 5'-end of the probe. Thus, a longer wavelength emission from acceptor might allow the probe to be used for SNP typing in chip based...
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