Article
A new approach to SNP genotyping with fluorescently labeled mononucleotides.
Nucleic acids research - 15 Apr 2004
Takatsu Kyoko, Yokomaku Toyokazu, Kurata Shinya, Kanagawa Takahiro
Abstract excerpt
Fluorescence resonance energy transfer (FRET) is one of the most powerful and promising tools for single nucleotide polymorphism (SNP) genotyping. However, the present methods using FRET require expensive reagents such as fluorescently labeled oligonucleotides. Here, we describe a novel and cost-effective method for SNP genotyping using FRET. The technique is based on allele-specific primer extension using...
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