Article
Single-nucleotide polymorphism detection using nanomolar nucleotides and single-molecule fluorescence.
Analytical biochemistry - 1 Apr 2004
Twist Charles R, Winson Michael K, Rowland Jem J, Kell Douglas B
Abstract excerpt
We have exploited three methods for discriminating single-nucleotide polymorphisms (SNPs) by detecting the incorporation or otherwise of labeled dideoxy nucleotides at the end of a primer chain using single-molecule fluorescence detection methods. Good discrimination of incorporated vs free nucleotide may be obtained in a homogeneous assay (without washing steps) via confocal fluorescence correlation spectroscopy...
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