Article
SNPs typing by base-discriminating fluorescence DNA probe.
Nucleic acids symposium series (2004) - 1 Jan 2005
Okamoto Akimitsu, Tainaka Kazuki, Ochi Yuji, Saito Isao
Abstract excerpt
For the development of a novel SNP typing method using BDF (base-discriminating fluorescent) nucleosides in biological samples, we examined the detection of the single base alteration in BRCA1 gene with PCR products amplified by an asymmetric PCR. A combination of PyU- and PyC-containing BDF probes clearly facilitates the discrimination of not only A/G homozygous samples but also heterozygous samples. The present...
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