Article
SNP genotyping using microsphere-linked PNA and flow cytometric detection.
Cytometry. Part A : the journal of the International Society for Analytical Cytology - 1 Apr 2005
Rockenbauer Eszter, Petersen Kenneth, Vogel Ulla, Bolund Lars, Kølvraa Steen, Nielsen Kirsten Vang, Nexø Bjørn A
Abstract excerpt
BACKGROUND: Single nucleotide polymorphisms (SNPs) represent the most frequent form of genetic variations. Some of the most sensitive methods for SNP genotyping employ synthetic oligonucleotides, such as the peptide nucleic acid (PNA). We introduce a new method combining allele-specific hybridization, PNA technology, and flow cytometric detection. We tested the design by genotyping a Danish basal cell carcinoma...
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