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Article

Comprehensive sequence-to-function mapping of cofactor-dependent RNA catalysis in the glmS ribozyme.

Nature communications - 3 Apr 2020

Andreasson Johan O L, Savinov Andrew, Block Steven M, Greenleaf William J

Abstract excerpt

Massively parallel, quantitative measurements of biomolecular activity across sequence space can greatly expand our understanding of RNA sequence-function relationships. We report the development of an RNA-array assay to perform such measurements and its application to a model RNA: the core glmS ribozyme riboswitch, which performs a ligand-dependent self-cleavage reaction. We measure the cleavage rates for all...

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Identifiers

PMID: 32245964
DOI: 10.1038/s41467-020-15540-1
PMCID: PMC7125110

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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