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Article

Easy preparation of a large-size random gene mutagenesis library in Escherichia coli.

Analytical biochemistry - 1 Sept 2012

You Chun, Percival Zhang Y-H

Abstract excerpt

A simple and fast protocol for the preparation of a large-size mutant library for directed evolution in Escherichia coli was developed based on the DNA multimers generated by prolonged overlap extension polymerase chain reaction (POE-PCR). This protocol comprised the following: (i) a linear DNA mutant library was generated by error-prone PCR or shuffling, and a linear vector backbone was prepared by regular PCR;...

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PMID: 22659340
DOI: 10.1016/j.ab.2012.05.022

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