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Article

pH-dependent conformational changes in Escherichia coli dihydrofolate reductase revealed by Raman difference spectroscopy.

Biophysical journal - 1 Feb 1997

Chen Y Q, Kraut J, Callender R

Abstract excerpt

The catalytic site of all dihydrofolate reductases contains an invariant carboxylic acid, equivalent to Asp-27 in Escherichia coli dihydrofolate reductase (ecDHFR). It has been found that various kinetic and ligand binding properties of ecDHFR show a pH profile with a pKa of about 6.5. The group responsible for this pKa is often assumed to be carboxyl group of Asp-27. To determine the ionization state of this...

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Identifiers

PMID: 9017218
DOI: 10.1016/s0006-3495(97)78727-7
PMCID: PMC1185616

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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