Article
Role of plasmid multimers in mutation to tetracycline resistance.
Molecular microbiology - 1 Oct 1991
Boe L, Marinus M G
Abstract excerpt
As an additional system for analysing mutations that appear to be specifically induced or directed, we have used a plasmid that contains the mnt repressor gene inserted as an operon fusion with the tet gene of the plasmid pBR322. Thus, the mnt gene product acts as a negative transcriptional regulator of tet gene expression. Mutations inactivating the Mnt repressor are recessive while those destroying operator...
Topics
- Escherichia coli
- Genes, Bacterial
- Mutation
- Operon
- Promoter Regions, Genetic
- R Factors
- Tetracycline
- Tetracycline Resistance