Article
Observations on the formation of deletions on monomeric and dimeric plasmids in Escherichia coli.
Molecular microbiology - 1 Oct 1994
Ravn P, Givskov M, Eegholm K M, Madsen S M, Boe L
Abstract excerpt
We have studied the formation of spontaneous mutations on plasmids present in the monomeric and dimeric states in a recF strain of Escherichia coli. Two test systems were employed: (i) the precise excision of Tn5 from the tetA gene of the plasmid pBR322 and (ii) operator constitutive (Oc) mutations on the pBR322-derived plasmid pPY97. The rate of Oc mutations was increased by a factor of three when this plasmid...
Topics
- Antiporters
- Bacterial Proteins
- Base Sequence
- DNA Transposable Elements
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Plasmids