Article
Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single-strand binding protein.
Molecular microbiology - 1 Oct 1990
Carraway M, Rewinski C, Marinus M G
Abstract excerpt
Single-stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia coli mutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 isolates were single base changes including transition, transversion and frameshift mutations. The remaining 32 isolates...
Topics
- Base Sequence
- Chromosome Deletion
- DNA Polymerase III
- DNA Replication
- DNA Transposable Elements
- DNA, Bacterial
- DNA, Single-Stranded
- Escherichia coli
- Genes, Bacterial
- Molecular Sequence Data
- Mutation