Article
Construction and characterization of a highly efficient Francisella shuttle plasmid.
Applied and environmental microbiology - 1 Dec 2004
Maier Tamara M, Havig Andrea, Casey Monika, Nano Francis E, Frank Dara W, Zahrt Thomas C
Abstract excerpt
Francisella tularensis is a facultative intracellular pathogen that infects a wide variety of mammals and causes tularemia in humans. It is recognized as a potential agent of bioterrorism due to its low infectious dose and multiple routes of transmission. To date, genetic manipulation in Francisella spp. has been limited due to the inefficiency of DNA transformation, the relative lack of useful selective markers,...
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