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Article

Allelic exchange in Francisella tularensis using PCR products.

FEMS microbiology letters - 12 Dec 2003

Lauriano Crystal M, Barker Jeffrey R, Nano Francis E, Arulanandam Bernard P, Klose Karl E

Abstract excerpt

We describe here a technique for allelic exchange in Francisella tularensis subsp. novicida utilizing polymerase chain reaction (PCR) products. Linear PCR fragments containing gene deletions with an erythromycin resistance cassette insertion were transformed into F. tularensis. The subsequent ErmR progeny were found to have undergone allelic exchange at the correct location in the genome; the minimum flanking...

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PMID: 14680699
DOI: 10.1016/S0378-1097(03)00820-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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