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Article

Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensor.

Protein science : a publication of the Protein Society - 1 Nov 2007

Amiss Terry J, Sherman Douglas B, Nycz Colleen M, Andaluz Sandra A, Pitner J Bruce

Abstract excerpt

Periplasmic expression screening is a selection technique used to enrich high-affinity proteins in Escherichia coli. We report using this screening method to rapidly select a mutated D-glucose/D-galactose-binding protein (GGBP) having low affinity to glucose. Wild-type GGBP has an equilibrium dissociation constant of 0.2 microM and mediates the transport of glucose within the periplasm of E. coli. The protein...

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Identifiers

PMID: 17905834
DOI: 10.1110/ps.073119507
PMCID: PMC2211708

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