Article
Establishment of a tissue-specific RNAi system in C. elegans.
Gene - 1 Oct 2007
Qadota Hiroshi, Inoue Makiko, Hikita Takao, Köppen Mathias, Hardin Jeffrey D, Amano Mutsuki, Moerman Donald G, Kaibuchi Kozo
Abstract excerpt
In C. elegans, mosaic analysis is a powerful genetic tool for determining in which tissue or specific cells a gene of interest is required. For traditional mosaic analysis, a loss-of-function mutant and a genomic fragment that can rescue the mutant phenotype are required. Here we establish an easy and rapid mosaic system using RNAi (RNA mediated interference), using a rde-1 mutant that is resistant to RNAi....
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