Kinds and locations of mutations arising spontaneously in the coding region of the HPRT gene of finite-life-span diploid human fibroblasts.

doi:10.1007/BF01233170

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Article

Kinds and locations of mutations arising spontaneously in the coding region of the HPRT gene of finite-life-span diploid human fibroblasts.

Somatic cell and molecular genetics - 1 Sept 1991

McGregor W G, Maher V M, McCormick J J

Abstract excerpt

Spontaneous thioguanine-resistant mutants were derived from populations of finite-life-span, diploid human fibroblasts by means of a fluctuation analysis. cDNA was prepared from mutant HPRT mRNA and amplified by the polymerase chain reaction, and the sequence of the product was analyzed. Exon del...

Topics

Base Sequence
Cell Line
Drug Resistance
Exons
Fibroblasts
Humans
Hypoxanthine Phosphoribosyltransferase
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Thioguanine

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